How In Silico Screening Can Guide Product Development in Cosmetics

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What's the role of in silico screening in cosmetic product development? Read on to learn more about the benefits of in silico screening and how it works.
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The Relationship Between Microbiology Testing and the Skin Microbiota

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Microbiology testing is essential for substantiating microbiome-friendly and anti-microbial claims. Read on to find out how cosmetic microbiology testing is connected to the skin microbiome.

Using Reconstructed Human Epidermis model in Skin Microbiome Research

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Skin microbiome research is developing rapidly. Are you up to date? Find out why 3D skin models are an important development in skin microbiome research.

Canities and hair pigmentation

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Canities (hair greying) has a wide-ranging social and cosmetic impact. QIMA Life Sciences is focusing on its physiopathology, underlying causes and treatments.

Comparing skin characteristics and molecular markers of xerotic foot skin between diabetic and non-diabetic subjects: An exploratory study

Xerosis cutis of the feet is one of the most common skin conditions among type 2 diabetics.
in vitro and ex vivo assays: skin barrier and hydration

Cosmetics Claims Substantiation: What You Need to Know

Profiling TLR agonist activity in keratinocytes and link to bacteria-induced innate immunity activation

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In this study, our goal was first to functionally profile and characterize the response of normal human keratinocytes (NHEK) to microbial patterns (TLR agonists) and IL-1α in parallel. To do so, the effects of multiple TLR agonists and IL-1α were evaluated on the gene expression profile, on signal transduction (NF-ƘB translocation) and also on IL-8 and IL-6 release. Secondly, we also tried to link these responses to those of pathogens of interest in skin microbiota (namely, C. acnes, S. aureus and P. aeruginosa), by evaluating the effect on IL-8 release by NHEK infected with living bacteria from these 3 strains...
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In Vitro Testing in Cosmetics: Pros and Cons

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How are In Vitro Testing Methods Used for Cosmetics?

With the growing distaste and outright bans of animal testing of cosmetic products, more and more cosmetics companies are turning to in vitro testing. Let’s take a closer look at the nature of in vitro testing and the opportunities it offers for cosmetics brands and manufacturers.

Facial skin mapping: from single point bio‐instrumental evaluation to continuous visualization of skin hydration, barrier function, skin surface pH, and sebum in different ethnic skin types

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Behavior of the two circulating androgens testosterone and DHEA and their main intracellular metabolites in an androgen-sensitive sebocyte cell line.

Androgens are known to strongly regulate the sebaceous gland. Relationships with testosterone have been reported and little is known about the role of DHEA. We showed that the most potent intracellular androgen metabolite, dihydrotestosterone (DHT), induced AR translocation, specific transcripts such as ras-related dexamethasone induced 1 (RASD1) and lipogenesis/lipid storage. We used these parameters to assess the behaviour and activity of the two main circulating androgens TESTO and DHEA, in comparison with DHT, in this model.

Innate immunity activation of sebocyte cells by living bacteria. Evidence of a potential immunosuppressive effect of DHT.

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Acne is a skin pathology targeting the pilosebaceous unit…sebum hypersecretion and bacterial infection. We studied the effects of living bacteria on the induction of an innate immune response in a sebocyte cell line.

Pharmacological effects of anti-androgens and other compounds in an androgen-sensitive sebocyte cell line.

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We analysed the activity of reference anti-androgens (Finasteride, Dutasteride, Cyproterone acetate), at the level of their potential targets (5-alpha-reductase, AR translocation, induced transcripts) and in a functional lipid accumulation assay. We also evaluated this androgen-induced lipid accumulation assay as a potential pharmacological tool to detect potential inhibitors from both androgenic and non-androgenic origins.

Androgens activate lipogenesis through an AKT-independent mTOR pathway stimulation and a limitation of autophagy in an androgen-sensitive sebocyte cell line.

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Androgens are key regulators of sebaceous function... A link between mTOR and androgen signaling has previously been reported...as well as a link between these processes and lipid synthesis...Here we aimed at confirming that DHT-induced lipid synthesis/accumulation is at least in part dependent on mTOR activation in the SEBO662AR cell line and that the autophagic process is consequently modified by the androgenic treatment.
in vitro and ex vivo assays: skin protection and immune defense system

Pollution and epigenetics: Histone H3 modifications in keratinocytes after exposure to urban dust and Benzo(a)pyrene

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In this work, human epidermal keratinocytes were used to study the effects of urban dust and benzo(a)pyrene on the epigenetic pattern of the histone H3 protein. The assay was then used to screen a series of plant extracts in order to find cosmetic actives to protect the skin against air pollution.
Dog Skin - Epidermal Cornification Proteins

Refined Immunochemical Characterization in Healthy Dog Skin of the Epidermal Cornification Proteins, Filaggrin, and Corneodesmosin

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Filaggrin (FLG) and corneodesmosin (CDSN) are two key proteins of the human epidermis.