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Assays for Hallmark Features of Cancer
Cytotoxicity profiling
- LDH release
- MTT reduction
- Annexin V / PI
- High content assay
Cell proliferation
- [3H]-Thymidine incorporation
- BrdU incorporation
- Ki-67-labeling
Signal transduction
- Protein phosphorylation
- Protein localization
- Second messengers
Vascularization assessment
- VEGF release
- Adhesion proteins expression
- Cell tube formation
DNA damage (H2A.X, 8-OH-dG)
Cell migration & invasion
Biomarker identification & validation
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oncology-related assays
Assays for Immune Cells in the Tumor Microenvironment (TEM)
Immune cells:
- Neutrophils
- Basophils
- Eosinophils
- Monocytes
- Macrophages
- Dendritic cells
- T cells
Assays:
- PMN, Macrophage, Dendritic Cell activation
- Phagocytosis
- PMN oxidative burst
- M1 or M2 polarization and switch
- Chemotaxis
- T cell differentiation
- Treg phenotyping
- Proliferation
- Cytokine and chemokine release