Behavior of the two circulating androgens testosterone and DHEA and their main intracellular metabolites in an androgen-sensitive sebocyte cell line.
Acne, Sebaceous gland regulationAndrogens are known to strongly regulate the sebaceous gland. Relationships with testosterone have been reported and little is known about the role of DHEA. We showed that the most potent intracellular androgen metabolite, dihydrotestosterone (DHT), induced AR translocation, specific transcripts such as ras-related dexamethasone induced 1 (RASD1) and lipogenesis/lipid storage. We used these parameters to assess the behaviour and activity of the two main circulating androgens TESTO and DHEA, in comparison with DHT, in this model.
Innate immunity activation of sebocyte cells by living bacteria. Evidence of a potential immunosuppressive effect of DHT.
Acné, Acne, Microbiome cutané, Microbiome cutané, Microbiome cutané, Sebaceous gland regulationAcne is a skin pathology targeting the pilosebaceous unit…sebum hypersecretion and bacterial infection. We studied the effects of living bacteria on the induction of an innate immune response in a sebocyte cell line.
Pharmacological effects of anti-androgens and other compounds in an androgen-sensitive sebocyte cell line.
Acne, Acné, Sebaceous gland regulationWe analysed the activity of reference anti-androgens (Finasteride, Dutasteride, Cyproterone acetate), at the level of their potential targets (5-alpha-reductase, AR translocation, induced transcripts) and in a functional lipid accumulation assay. We also evaluated this androgen-induced lipid accumulation assay as a potential pharmacological tool to detect potential inhibitors from both androgenic and non-androgenic origins.
Androgens activate lipogenesis through an AKT-independent mTOR pathway stimulation and a limitation of autophagy in an androgen-sensitive sebocyte cell line.
Acne, Acné, Peau grasse, hyperséborrhée et séborégulation, Sebaceous gland regulationAndrogens are key regulators of sebaceous function... A link between mTOR and androgen signaling has previously been reported...as well as a link between these processes and lipid synthesis...Here we aimed at confirming that DHT-induced lipid synthesis/accumulation is at least in part dependent on mTOR activation in the SEBO662AR cell line and that the autophagic process is consequently modified by the androgenic treatment.
Effects of different kinds of pollutants on the lipidic metabolism of human sebocytes and protective effects of a new macroalgae culture extract
Cosmetics, Peau grasse, hyperséborrhée et séborégulation, Protection et défenses cutanées, Sebaceous gland regulation, Skin protection and immune defense systemSebocytes lipid production are stimulated by pollutants. Ame acts by protecting epidermal cells, human sebocytes and skin from urban dust.
Androgens induce sebaceous differentiation in sebocyte cells expressing a stable functional androgen receptor
Acné, Acne, Cell and tissue engineering, Ingénierie cellulaire et tissulaire, Sebaceous gland regulation, Vieillissement cutanéIn order to study the impact of active androgens in sebocytes, we constructed a stable human sebocyte cell line derived from SEBO662 [17] constitutively expressing a fully functional AR. In these SEBO662 AR+ cells, dihydrotestosterone (DHT) induced AR nuclear translocation and the strong modulation of a set of transcripts (RASD1, GREB1...) known to be androgen-sensitive in other androgenic cells and tissues.
Immortalized sebocytes SEBO662 can spontaneously differentiate into a sebaceous-like phenotype when cultured as a 3D epithelium
Acne, Acné, Cell and tissue engineering, Ingénierie cellulaire et tissulaire, Sebaceous gland regulationSEBO662 multilayers spontaneously differentiate into a sebaceous-like structure as shown by the strong polarized expression of the late sebaceous marker EMA, the overexpression of some lipogenic markers and lipid production on the upper side of the epithelium.