Labeling of normal human dermal fibroblasts (Myosin in green, actin in blue, nuclei in red)

Normal Human Dermal Fibroblasts (NHDF) in In Vitro Research

What are the applications of Normal Human Dermal Fibroblasts and how are they used in various studies? Learn about NHDF and its applications in this overview.

Immunolabeling

QIMA Life Sciences offers immunolabeling (immunofluorescence & immunohistochemistry) and research & valdiation of antibody services.
Skin surface with hair_SEMX6000

Electron microscopy service – TEM / SEM

Slide scanning

Example of Metabolic GAINS Profile

Phytochemical analysis & metabolic profiling – GAINS

GPDB: Chemical Compound Structure Database

Ethnopharmacologic database -GPDB

in silico screening – SELNERGY

Sample and slide preparation

Human-skin

Histological staining

Development of a new model of reconstituted mouse epidermis and characterization of its response to proinflammatory cytokines

The development of three-dimensional models of reconstituted mouse epidermis (RME) has been hampered by the difficulty to maintain murine primary keratinocyte cultures and to achieve a complete epidermal stratification. In this study, a new protocol is proposed for the rapid and convenient generation of RME,
ageing reconstructed epidermis

Development of a new model of reconstructed aged skin useful to study antiageing effects of cosmetic compounds

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The development of new anti-ageing products needs performant in vitro models mimicking morphological changes and physiological modifications appearing during skin ageing. In order to have access to a simple model mimicking the epidermis ageing but in relation with a normal dermis, we have developed a new in vitro model of reconstructed skin comprising an aged epidermis covering a reconstructed dermis built with collagen and normal (young) fibroblasts.

Androgens induce sebaceous differentiation in sebocyte cells expressing a stable functional androgen receptor

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In order to study the impact of active androgens in sebocytes, we constructed a stable human sebocyte cell line derived from SEBO662 [17] constitutively expressing a fully functional AR. In these SEBO662 AR+ cells, dihydrotestosterone (DHT) induced AR nuclear translocation and the strong modulation of a set of transcripts (RASD1, GREB1...) known to be androgen-sensitive in other androgenic cells and tissues.

Human embryonic stem-cell derivatives for full reconstruction of the pluristratified epidermis: a preclinical study

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To assess whether the keratinocyte progeny of human embryonic stem cells (hESCs) could be used to form a temporary skin substitute for use in patients awaiting autologous grafts, we investigated the cells' capability of constructing a pluristratified epidermis.