Our Models & Assays To Address Hair Growth
MODELS
QIMA Life Sciences has developed several in vitro and ex vivo models to help you evaluate your compound or finished product.
2D In vitro models:
- Human follicle dermal papilla fibroblasts (HFDPC)
- Outer root sheath keratinocytes (ORSK)
3D In vitro models:
- Dermal papilla (DP) spheroids
- Hair follicle organoids (DPF-NHEK/ORSK organoids)
Ex vivo models:
- Hair follicle organ culture
- Scalp skin organ culture
All our models can be treated with various extrinsic stresses:
- Dust (pollution)
- UVA and UVB (UV radiation)
- H2O2 (oxidative stress)
READOUT PARAMETERS
We evaluate the following standard readout parameters by morphology, immunohistology, and quantitative (immuno-)histomorphometry:
- Macroscopic hair cycle staging (number of hair follicle in anagen VI and catagen)
- Hair follicle elongation (correlates with hair shaft production)
- Hair matrix keratinocyte proliferation and apoptosis
- Microscopic hair cycle staging and score (number of hair follicles in anagen VI, early, mid, and late catagen)
- Hair cycle independent hair follicle pigmentation
- Expression of growth factors regulating hair cycle
In addition, using RNAseq, qRT-PCR, and/or in situ hybridization, we can analyze:
- Expression of molecules involved in anagen/catagen convertion
Also, we can assess these within specific compartments from skin or hair tissue sections following laser capture microdissection.
* Additional readout parameters are available, and customized experiments can be designed to meet the needs of our customers.
Scientific Publications on Hair Growth
Platt et al., 2022, Bertolini et al., 2020, Jimenez et al., 2020, Szabó et al.,2020, Fischer et al., 2020, Hundt et al., 2020, Alam et al., 2020, Laufer etal., 2020, Lisztes et al., 2020, Chéret et al., 2018, Haslam et al., 2018, Alam et al., 2018, Parodi et al, 2018, Hawkshaw et al., 2018, Olah et al., 2016, Langan et al., 2015, Kloepper et al.,2010
Discover our Documentary Resources
Take a look at our
Hair Growth Flyer
Take a look at our
Hair Skinification Flyer
NEED MORE INFORMATION?
We are here to help!
