Types of treated samples
Bioalternatives can perform the RNA extraction step from:
- Cell culture (monolayers or suspension)
- Bacteria
- Reconstructed tissues in culture
- ex vivo models in culture
- Tests (blood, tissues)
Recommendations:
When sending samples, it is essential to take the necessary precautions so as to avoid degradation during transport; dry ice packaging is recommended. If sending tissues, preliminary RNAlater treatment is advised.
Extraction step
Bioalternatives can perform the total RNA extraction step of your samples in a standard way.
Bioalternatives can also perform extractions in a more specific way:
- miRNA
- mRNA
- DNA
Applications:
- RT-qPCR
- Transcriptome analysis by microarray
- miRNA expression analysis (RT-qPCR or microarray)
Quality control of RNA
The quality of extracted RNA depends on the conditions in which the samples have been prepared, collected and stored. Once the RNA extraction step is performed, samples require qualitative and quantitative control.
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- The quantity of extracted RNA is determined by spectrophotometry (Nanovue™ spectrophotometer, GE Healthcare)
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- The quality of extracted RNA is measured by capillary electrophoresis, using the Agilent® Bioanalyzer 2100. The level of RNA quality is linked to an INR value determined by the device: the closer this value is to 10, the better the RNA quality is.
