At Bioalternatives, we offer more than thirty standard (topographic) histological and special (descriptive) staining techniques.
These staining processes are carried out after a thorough preparation of samples and slides.
– With routine standard staining techniques, tissue morphology (structure, organization, dimension / nucleus, cytoplasm, collagen fibers) can be examined and tissue integrity or alteration can be evaluated.
– Special staining techniques allow us to go further in tissue observation and to visualize particular tissue components (lipids, melanin pigments, elastic fibers etc.).
Some tissue components can be highlighted in situ by using special staining techniques based on biochemical reactions between the dyes and the tissue components.
Please find below a list of available staining techniques. For any special staining request, please contact us.
This is a non-exhaustive list of the histological staining techniques we offer:
| STAINING | TISSUE COMPONENTS |
|---|---|
| Alcian blue | Mucosubstances / GAGs |
| Toluidine blue | Mastocytes |
| Fontana Masson | Melanin |
| Gordon Sweet | Reticulin fibres |
| Grocott | Fungal microorganisms |
| Hematoxyline/eosine | Nuclei, cytoplasmic structures |
| Hematoxyline/eosine/safran | Collagen |
| Herovici | Mature & immature collagen |
| Luna | Elastic fibres |
| Masson Trichrome | Collagen |
| Mowry | Mucosubstances/GAGs, collagen |
| Nile red | Lipids |
| STAINING | TISSUE COMPONENTS |
|---|---|
| Sudan Black | Lipids |
| Oil Red | Lipids |
| Orcein safran | Elastic fibres |
| PAS (Periodic Acid Schiff) | Glycogen, mucin, collagen |
| Silverman-Movat Pentachrome | Elastic fibres, muscle fibres, mucosubstances/GAGs, collagen |
| Alizarine Red | Calcium |
| Sirius Red | Collagen |
| Safranin-O | Cartilage |
| Van Gieson | Collagen |
| Von Kossa | Calcium |
| Warthin Starry | Melanin |
