2D In vitro models

2D in vitro models have been developed using 2D cell culture, a technique widely used in biological research.

These models are of great value for early drug discovery stages as well as for preclinical in vitro research in the context of cosmetic efficacy testing.

What Are 2D In Vitro Models?

A 2D in vitro model is a two-dimensional cell culture system in which adherent cells are grown on a flat surface.

In such models, cells can be cultured on plastic or glass Petri dishes, flasks, or plates.

Adherent cells only grow when attached to a surface; therefore, culture vessels require specific surface treatments to promote cell adhesion. Typically, vessels are treated to be hydrophilic and negatively charged or coated with specific proteins such as collagen, fibronectin, laminin, or poly-lysine to enhance cell adhesion.

When cultured in the appropriate medium, adherent cells grow as a monolayer, forming a two-dimensional structure.

Our 2D In Vitro Models

Immortalized or transfected cells
Primary cells
Progenitor cells
Co-cultures

The donor’s genetic background and health status can influence the characteristics of primary cells, while culture conditions can affect the characteristics both primary cells and cell lines.

Our models can be thus categorized as:

  • “healthy”,
  • “pathological”, and
  • “mimicking pathological conditions”.

Our Extensive Array Of Assays

We provide a wide range of assays on various 2D in vitro models. 

Here are some examples of readouts we can assess: 

  • Cytotoxicity
  • Oxidative stress markers
  • Inflammation markers
  • Cell proliferation
  • Signal transduction
  • DNA damage
  • Cell migration
  • Cell differentiation
  • Melanogenesis
  • Lipidogenesis
  • …among many others!

Why Use 2D In Vitro Models?

2D cell culture technology has been a cornerstone of laboratory research for decades. Here are some reasons for its enduring popularity:

  • Lower cost: compared to other systems, 2D cell culture systems cost less, partly due to economies of scale
  • Faster use: 2D cell culture can be quickly used for testing and experimentation, in comparison to 3D cultures
  • Easier experimentation and analysis: 2D cell cultures are generally simpler to analyze, measure, and manipulate than 3D models
  • Higher throughput: the easily miniaturized format of 2D cultures makes them better suited for testing a large number of experimental condition
  • Tradition and familiarity: 2D cell culture is a fundamental technique in laboratory research, making it familiar for many researchers.
  • Extensive literature: because most previous studies have traditionally used 2D cell cultures, it is easier to compare new results with established historical data

You want to learn more about our 2D in vitro models?
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