TECHNICAL FOCUS:
Vitiligo-induced ex vivo model
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Key takeaway:
This model offers a robust platform for early-stage vitiligo drug screening and mechanistic studies.
A comprehensive understanding of the biological mechanisms underlying vitiligo is critical for the development of clinically predictive models that accurately recapitulate the pathophysiological features of human skin. Such models represent a valuable alternative to conventional diseased skin organ cultures (ex vivo systems), particularly in addressing the limited availability of patient-derived biopsy samples.
Our team has established a vitiligo-induced ex vivo skin model utilizing a proprietary inducer cocktail that reliably elicits hallmark features of the disease, including inflammation, elevated oxidative stress, and melanocytic loss.
The development of this model was based on previously published studies (Hariharan et al., 2010; Kim et al., 2016) and proprietary data generated at QIMA Monasterium.
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Vitiligo
Melanocytic loss
Test: Melanocyte quantification
Method: Immunohistochemistry
Observation: Treatment with the proprietary vitiligo-inducing cocktail results in a reduction of epidermal melanocytes (MITF⁺ cells).
T-cell enrichment
Test: T cell quantification
Method: Immunohistochemistry
Observation: Treatment with the proprietary vitiligo-inducing cocktail results in an increase of T cells (CD3⁺ cells).
