Immunomarquages

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QIMA Life Sciences réalise des prestations d'immunomarquage (immunofluorescence et immunohistochimie), de validation d'anticorps et de co-marquages.

Canitie et pigmentation des cheveux

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La canitie (grisonnement des cheveux) a un large impact social et cosmétique. QIMA Life Sciences présente ici sa physiopathologie, ses causes sous-jacentes et ses traitements.
Skin surface with hair_SEMX6000

Profiling TLR agonist activity in keratinocytes and link to bacteria-induced innate immunity activation

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In this study, our goal was first to functionally profile and characterize the response of normal human keratinocytes (NHEK) to microbial patterns (TLR agonists) and IL-1α in parallel. To do so, the effects of multiple TLR agonists and IL-1α were evaluated on the gene expression profile, on signal transduction (NF-ƘB translocation) and also on IL-8 and IL-6 release. Secondly, we also tried to link these responses to those of pathogens of interest in skin microbiota (namely, C. acnes, S. aureus and P. aeruginosa), by evaluating the effect on IL-8 release by NHEK infected with living bacteria from these 3 strains...
Quantification of the IL-1 family member proteins in keratinocyte culture medium (A) and in keratinocyte extract (B)

Fibroblasts are potentially the key sensors of epidermis lesion through keratinocyte IL-1 signalling

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Our aim was to revisit the primary sterile epidermis lesion considering the alarmins constitutively expressed in the skin tissue and notably the IL-1 family members (IL-1fm) released by lesional keratinocytes. At homeostasis, the non-lesional epidermis constitutes a barrier against various external aggressions...
GPDB: Base de donnée de molécules naturelles

Base de données ethnopharmacologique – GPDB

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La GPDB est une base de données ethnopharmacologique innovante et robuste. Elle est le fruit de nombreuses années de recherches couplées à la littérature scientifique. En effet, cette base de données rassemble les résultats de tests biologiques menés sur des extraits de plantes et des molécules isolées ainsi que les données issues de nombreux brevets, de revues scientifiques et de la pharmacopée mondiale.
Profiling phytochimique et métabolique

Facial skin mapping: from single point bio‐instrumental evaluation to continuous visualization of skin hydration, barrier function, skin surface pH, and sebum in different ethnic skin types

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Dry skin is one of the most important concerns of consumers worldwide. Despite huge efforts over several decades, the personal care industry still does not offer a perfect solution to satisfy the unmet needs of consumers for moisturising treatments in different ethnic groups.
Herovici staining (x40)

Behavior of the two circulating androgens testosterone and DHEA and their main intracellular metabolites in an androgen-sensitive sebocyte cell line.

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Androgens are known to strongly regulate the sebaceous gland. Relationships with testosterone have been reported and little is known about the role of DHEA. We showed that the most potent intracellular androgen metabolite, dihydrotestosterone (DHT), induced AR translocation, specific transcripts such as ras-related dexamethasone induced 1 (RASD1) and lipogenesis/lipid storage. We used these parameters to assess the behaviour and activity of the two main circulating androgens TESTO and DHEA, in comparison with DHT, in this model.

Behavior of the two circulating androgens testosterone and DHEA and their main intracellular metabolites in an androgen-sensitive sebocyte cell line.

Androgens are known to strongly regulate the sebaceous gland. Relationships with testosterone have been reported and little is known about the role of DHEA. We showed that the most potent intracellular androgen metabolite, dihydrotestosterone (DHT), induced AR translocation, specific transcripts such as ras-related dexamethasone induced 1 (RASD1) and lipogenesis/lipid storage. We used these parameters to assess the behaviour and activity of the two main circulating androgens TESTO and DHEA, in comparison with DHT, in this model.

Innate immunity activation of sebocyte cells by living bacteria. Evidence of a potential immunosuppressive effect of DHT.

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Acne is a skin pathology targeting the pilosebaceous unit…sebum hypersecretion and bacterial infection. We studied the effects of living bacteria on the induction of an innate immune response in a sebocyte cell line.