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HYDRATION AND SKIN BARRIER

Skin hydration partly determines the aspect of the skin (dry, rough and oily skin, etc.). Through its barrier function, skin is our shield from external stress factors and prevents the evaporation of such essential elements as water, ions and amino acids.

Skin hydration is mostly linked to the skin barrier function. The role of a moisturizing skin care product is to act on skin surface to physically limit water loss (occlusive effect) or to act at the cellular level via different biological action models:

  • reinforcement of the barrier function and of epidermal differentiation
  • restoration of skin lipids
  • stimulation of hygroscopic endogenous factors (water retention)
  • improvement of cellular exchanges in solutes and in water

skin proteic structure, physico biochemical barrier

Hydration is a multifactorial process that cannot be understood through a single assay or model. It is therefore more conclusive to use a panel of complementary efficacy assays in order to study and demonstrate the hydrating properties of an active compound or cosmetic product.

Webinar

skin barrier and hydration

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Hydration & skin barrier: available models and assays

in silico assays

in vitro
and ex vivo
assays & hydration

Biochemical analysis
& hydration

Clinical imaging
& hydration

in vitro models & assays

Models

Qima Life Sciences has many in vitro or ex vivo models at your disposal:

  • normal human epidermal keratinocytes (NHEK)
  • normal reconstructed human epidermis (RHE) & Reconstructed human epidermis in default
  • sebocytes (SEBO662AR)
  • full thickness skin (FTSK)
  • skin explants (ex vivo)
Filaggrin labeling in normal human keratinocytes
SEBO662 (lipids in green, cytokeratin 7 in red)
Kallikrein-5 labeling in RHE
Loricrin labeling in full thickness skin
Caspase-14 labeling in Skin explant
EMA immunolabeling in 3D sebocyte model
Evaluation of skin barrier and hydration

We can evaluate case by case the hydrating effect of active compounds or formulations by measuring:

  • the reinforcement of the barrier function
  • the stimulation of the epidermal differentiation (e.g. filaggrin, involucrin, transglutaminase, cytokeratins)
  • lipid synthesis (acid mantle):
    • sebaceous lipids
    • epidermal lipids (e.g. ceramides, cerebrosides and phospholipids)
  • the expression or synthesis of epidermal extracellular matrix components:
    • glycosaminoglycans and hyaluronic acid
    • proteoglycans and ECM receptors
    • proteases (e.g. MMPs)
  • the expression of markers of epidermal cohesion and intercellular cell junctions:
    • occluding junctions and attachment proteins (claudin, occludin, desmogleins, etc.)
    • dermoepidermal junction (e.g. integrin V, collagen IV, collagen VII, etc.)
    • gap junctions (e.g. connexins) and molecular channels (e.g. aquaporins)
skin physico biochemical barrier evaluation

Here are a few examples among all standard assays proposed by Qima Life Sciences in the field of hydration and reinforcement of the barrier function:

  • Claudin-1-expression#Ref

    NHEK, desmosomal, tight & other cell-cell adhesion proteins (basal)

    1.00€
    Add to cart Show Details
  • Calcium-induced gene expression

    NHEK, epidermal barrier markers (mRNA) (basal)

    1.00€
    Add to cart Show Details
  • Hyaluronic acid synthesis

    NHEK, hyaluronic acid synthesis / release (basal)

    1.00€
    Add to cart Show Details
  • Ceramides and cerebrosides synthesis

    RHE, phospholipid & ceramide neosynthesis (basal)

    1.00€
    Add to cart Show Details
  • Caffein diffusion

    RHE, transepidermal permeation and barrier function (basal)

    1.00€
    Add to cart Show Details

Biochemical analysis of non-invasive clinical samples

Analysis of lipids involved in the barrier function

Our company has developed ready-to-use non-invasive collection kits to analyze the lipids and biomarkers of the skin surface from your samples or from those of your clinical center.

The epidermal lipids involved in the barrier function of the epidermis (ceramides, fatty acids and cholesterol) are removed using the SW Kit.

The analysis of these lipids makes it possible to evaluate the quality of the intercorneocyte cement involved in the barrier function of the epidermis and in the prevention of transepidermal water loss (TEWL).

These evaluations help support your claims about the efficacy of biomimetic products, barrier products, protective products, moisturizers, etc.

Analysis of the components of the Natural Moisturizing Factor (NMF)

The amino acids and minerals present on the surface of the skin are collected using the SW Kit:

  • PCA / UCA (cis/trans) – (catabolites of filaggrin)
  • Amino acids
  • Urea, lactates
  • Mineral elements: Ca, K, Na, Mg, Zn, etc.

The analysis of these compounds makes it possible to evaluate the impact of the NMF component on skin hydration.

These analysis help support your claims about the efficacy of hygroscopic products, barrier products, protective products, moisturizers, etc.

Screening des céramides

Ceramide screening – LC/MS

–

Cornéocytes endommagés et sains - SEMX500

Damaged and healthy corneocytes – SEMX500

–

Analyse des PCA - LC/UV

PCA analysis – LC/UV

Data mapping and clinical imaging

Measurement of the degree of hydration of the skin and 3D mapping

The function of the skin barrier and the degree of hydration (in outer skin layers) can be measured in vivo by using noninvasive methods, such the Tewameter and Corneometer (Courage & Khazaka) and the VapoMeter (Delfin). These devices provide information on the evolution of the water content of the stratum corneum and of the skin barrier function over time, by instrumentally measuring the electrical capacity of the skin and transepidermal water loss.
The evolution over time of this data on face, hand and body skin can be modeled by using the smart mapping solutions developed by Newtone Technologies.
Measurements are performed in several locations of the selected skin area. The mean values are then transformed into colored surfaces, according to a reference color bar, and mapping algorithms are used to visualize the change in hydration under treatment (high to low values of either electric capacity or skin water loss).
These mappings can be displayed on different visual templates, such as a 3D face, average faces, or real photographs, and can show the skin improvement caused by the use of an active ingredient or cosmetic product.
These solutions can be offered to illustrate the results obtained by the bioanalysis of noninvasive clinical samples (mapping of epidermal lipid changes over time, mapping of NMF compounds under cosmetic treatment).

Hydration map, obtained from corneometric data, by using an avatar as a 3D model of a Caucasian face

Measurement of roughness of skin pattern

Dry skin and skin with an altered barrier function can present common visual characteristics.
From a clinical point of view, dry skin appears coarser than normal skin, and, according to the degree of dryness, can even present visible squamae.
Dry skin can be associated to feelings of discomfort, but there can also be some visible irritation, due to an underlying skin affliction or to scratching.
In addition to the discomfort caused by skin dryness, the associated roughness, dull aspect, and uneven texture can become unsightly. In vivo skin imaging allows the evolution or roughness and color parameters to be measured over time.
Skin roughness can be analyzed objectively and quantitatively by measuring the parameters of the fine and isotropic microrelief skin pattern.
This pattern is composed of polygons, mainly triangles and quadrangles, delimited by folds, and their characteristics evolve under the effect of moisturizing creams. These folds are classified according to their length and their depth. The folds that are most visible to the naked eye are primary lines, which are wide and deep (30-100µm deep compared to skin surface), while secondary lines are not as deep (5-40µm) and narrower.
Newtone Technologies has developed a portable camera, SkinCam, which is connected to storage and data analysis servers in real time, and which, combined with software suites and 3 D image analysis, can be used to measure the skin pattern characteristics and roughness.
Images can be acquired in a laboratory or at home, by the subjects themselves, and provide high-resolution information on the different skin microrelief parameters and their evolution due to hydration.

Measurement of dryness and irritation of hand skin, by using the SkinCam, and microrelief analysis in 3 D (before treatment, after 1 day and 7 days of treatment)

Find out about all our standard assays - Direct access to catalog
A project, an idea or a question? Contact us & let’s talk about it!

Hydration and skin barrier: posts and publications

Dog Skin - Epidermal Cornification Proteins

Refined Immunochemical Characterization in Healthy Dog Skin of the Epidermal Cornification Proteins, Filaggrin, and Corneodesmosin

Barrière cutanée et hydratation, Médecine vétérinaire, Skin barrier and hydration, Veterinary medicine

Filaggrin (FLG) and corneodesmosin (CDSN) are two key proteins of the human epidermis.

Read more
https://qima-lifesciences.com/wp-content/uploads/2021/08/mw_Dog-Skin-Epidermal-Cornification-Proteins.png 368 655 Julie https://qima-lifesciences.com/wp-content/uploads/2021/10/QIMA_life_sciences@4x1.png Julie2019-02-01 13:14:402021-08-30 13:14:58Refined Immunochemical Characterization in Healthy Dog Skin of the Epidermal Cornification Proteins, Filaggrin, and Corneodesmosin
Rab11

Rab11a Is Essential for Lamellar Body Biogenesis in the Human

Barrière cutanée et hydratation, Skin barrier and hydration

Most of the skin barrier function is attributable to the outermost layer of the epidermis, the stratum corneum, which is composed of flattened, anucleated cells called corneocytes surrounded by a lipid-enriched lamellar matrix.

Read more
https://qima-lifesciences.com/wp-content/uploads/2021/08/mw_Rab11.png 368 655 Julie https://qima-lifesciences.com/wp-content/uploads/2021/10/QIMA_life_sciences@4x1.png Julie2016-06-01 14:32:342021-08-30 14:33:00Rab11a Is Essential for Lamellar Body Biogenesis in the Human

 

 

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  • La base légale du traitement est l’intérêt légitime du QIMA Life Sciences. Les données collectées seront communiquées aux seuls destinataires suivants : Les services concernés et habilités par QIMA Life Sciences. Les données sont conservées pendant une durée de 3 ans à compter de la collecte ou de votre dernier contact entrant.

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    N.B : Les catégories de données suivantes ; NOM / SOCIETE / EMAIL et PAR QUEL TYPE DE SERVICE ÊTES VOUS INTERESSE sont obligatoires. La non-fourniture de ces informations entrainera l’impossibilité pour QIMA Life Sciences de vous répondre.

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Address

QIMA Life Sciences
Headquarters
1bis rue des plantes - CS 50011
86160 Gençay - France
Tel. +33 (0) 5 49 36 11 37
Mail: ls@qima.com

International offices
France - Labège, Lyon
Germany - Münster
USA - Buffalo, NY
Brazil - Botucatu, SP

Latest posts

  • Performance and Tolerance of a Protocol for Idiopathic Chronic Greasy Seborrhea in 18 Dogs Using a Shampoo and Mousse Containing Plant Extracts
  • Evaluation of a new connected portable camera for the analysis of skin microrelief and the assessment of the effect of skin moisturisers
  • Clinical and Microbiological Performances and Effects on Lipid and Cytokine Production of a Ceruminolytic Ear Cleaner in Canine Erythemato-Ceruminous Otitis Externa

Cosmetic

  • Hydration and skin barrier
  • Epidermal regeneration
  • Skin firmness & cohesion
  • Skin ageing
  • Skin protection & defense
  • Skin pigmentation
  • Oily skin and hyperseborrhea
  • Skin microcirculation and vascularization
  • Slimming and adipocyte metabolism
  • Hair growth and alopecia

Pharmacology

  • Immuno-Inflammation
  • Neurobiology
  • Veterinary medicine

Dermatology

  • Acne
  • Hair growth and alopecia
  • Atopic dermatitis
  • Psoriasis
  • Wound healing and skin regeneration
  • Melanoma
  • Vitiligo (coming soon)
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  • Les informations recueillies sur ce formulaire sont enregistrées dans un fichier informatisé par QIMA LIFE SCIENCES pour vous contacter afin de répondre à votre demande et vous aider à sélectionner le ou les tests les mieux adaptés à votre projet.

  • La base légale du traitement est l’intérêt légitime du QIMA Life Sciences. Les données collectées seront communiquées aux seuls destinataires suivants : Les services concernés et habilités par QIMA Life Sciences. Les données sont conservées pendant une durée de 3 ans à compter de la collecte ou de votre dernier contact entrant.

    Vous pouvez accéder aux données vous concernant, les rectifier, demander leur effacement ou exercer votre droit à la limitation du traitement de vos données, retirer votre consentement au traitement de vos données à tout moment, vous opposer au traitement de vos données et vous disposez d’un droit à la portabilité de vos données.

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    Pour exercer ces droits vous pouvez nous contacter à l’aide du lien suivant Gestion des droits ou en écrivant à dpo-qima@agencergpd.eu.

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  • Les informations recueillies sur ce formulaire sont enregistrées dans un fichier informatisé par QIMA LIFE SCIENCES pour vous contacter afin de répondre à votre demande et vous aider à sélectionner le ou les tests les mieux adaptés à votre projet.

  • La base légale du traitement est l’intérêt légitime du QIMA Life Sciences. Les données collectées seront communiquées aux seuls destinataires suivants : Les services concernés et habilités par QIMA Life Sciences. Les données sont conservées pendant une durée de 3 ans à compter de la collecte ou de votre dernier contact entrant.

    Vous pouvez accéder aux données vous concernant, les rectifier, demander leur effacement ou exercer votre droit à la limitation du traitement de vos données, retirer votre consentement au traitement de vos données à tout moment, vous opposer au traitement de vos données et vous disposez d’un droit à la portabilité de vos données.

    Consultez le site cnil.fr pour plus d’informations sur vos droits.

    Pour exercer ces droits vous pouvez nous contacter à l’aide du lien suivant Gestion des droits ou en écrivant à dpo-qima@agencergpd.eu.

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