Alternative (non-animal) methods for cosmetic testing: current status and future prospects – UV-induced effects

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Cosmetic ingredients and mixtures of ingredients absorbing UV light (in particular UV filter chemicals used, for example, to ensure the light stability of cosmetics or used in sun protection products) should be tested for acute phototoxic and photogenotoxic potential.
acute toxicity

Alternative (non-animal) methods for cosmetic testing: current status and future prospects – Acute toxicity

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From the regulatory point of view, the main objective of acute toxicity testing is basically to classify chemicals according to their intrinsic toxicity
lipidocolloid dressing

Stimulation of the proliferation of human dermal fibroblasts in vitro by a lipidocolloid dressing

The effect of Urgotul on normal human dermal fibroblast proliferation was studied in vitro and compared with that of two other dressing: Mepitel and Tulle Gras.

IL-22 inhibits epidermal differentiation and induces proinflammatory gene expression and migration of human keratinocytes

IL-22 belongs to a family of cytokines structurally related to IL-10, including IL-19, IL-20, IL-24, and IL-26. In contrast to IL-10, IL-22 has proinflammatory activities. IL-22 signals through a class II cytokine receptor composed of an IL-22-binding chain, IL-22RA1, and the IL-10RB subunit, which is shared with the IL-10R.
gene expression

Comparison of gene expression profiles in human keratinocyte mono-layer cultures, reconstituted epidermis and normal human skin; transcriptional effects of retinoid treatments in reconstituted human epidermis

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In order to validate a model for predictive screening of dermatological drugs, we used a customized cDNA macro-array system containing 475 skin-related genes to analyze the gene expression patterns in human keratinocytes from different origins.

Development of a highly sensitive in vitro phototoxicity assay using the SkinEthic reconstructed human epidermis

The reconstituted human epidermis model SkinEthic was used to evaluate the phototoxicity of topically applied chemicals.

Expression of type 1 5-alpha-reductase and metabolism of testosterone in reconstructed human epidermis

We analysed the potential of a human epidermal tissue reconstituted by cell culture (RHE, provided by SkinEthic Laboratories, Nice, France) as a model for assessing 5-alphaR activity.