Rab11

Rab11a Is Essential for Lamellar Body Biogenesis in the Human

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Most of the skin barrier function is attributable to the outermost layer of the epidermis, the stratum corneum, which is composed of flattened, anucleated cells called corneocytes surrounded by a lipid-enriched lamellar matrix.

Gene expression

PCR arrays are dedicated to gene expression analysis by quantitative PCR. They are sets of primers selected according to a theme or a given biological process. Gene expression analysis by PCR arrays is considered as the most effective and cost-efficient method for studying a panel of targeted genes.

Microarrays and transcriptomic analysis

QIMA Life Sciences, an Affymetrix® service provider, offers full genome analysis by microarrays (GeneAtlas®platform).

Bioinformatics: basic and functional data processing

Bioinformatics services dedicated to transcriptomic data analysis and processing: raw data processing, quality control, normalization, statistical analysis, basic and advanced functional analysis (clusterization and pathway).
Scalp surface lipids

Exploration of scalp surface lipids reveals squalene peroxide as a potential actor in dandruff condition

Dandruff is a common but complex disorder with three major contributing factors: (1) individual predisposition, (2) scalp sebum and (3) Malassezia yeast colonization.
imiquimod

Imiquimod-induced skin inflammation in mice is dependent on IL-1R1 and MyD88 signaling but independent of the NLRP3 inflammasome

The pathogenesis of inflammatory skin diseases such as psoriasis involves the release of numerous proinflammatory cytokines, including members of the IL-1 family. Here we report overexpression of IL-1α, IL-1β, and IL-1 receptor antagonist mRNA, associated to expression of IL-23p19, IL-17A, and IL-22 in skin cells

Androgens induce sebaceous differentiation in sebocyte cells expressing a stable functional androgen receptor

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In order to study the impact of active androgens in sebocytes, we constructed a stable human sebocyte cell line derived from SEBO662 [17] constitutively expressing a fully functional AR. In these SEBO662 AR+ cells, dihydrotestosterone (DHT) induced AR nuclear translocation and the strong modulation of a set of transcripts (RASD1, GREB1...) known to be androgen-sensitive in other androgenic cells and tissues.

IL22/IL-22R pathway induces cell survival in human glioblastoma cells

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Interleukin-22 (IL-22) is a member of the IL-10 cytokine family that binds to a heterodimeric receptor consisting of IL-22 receptor 1 (IL-22R1) and IL-10R2. IL-22R expression was initially characterized on epithelial cells, and plays an essential role in a number of inflammatory diseases.

Study of proliferation and 3D epidermal reconstruction from foreskin, auricular and trunk keratinocytes in children

Our studies highlight the potential of foreskin tissue for autograft applications in boys. A suitable alternative donor site for autologous cell transplantation in female paediatric burn patients remains an open question in our department. We tested the hypothesis that in vitro studies and RHE reconstructive capacities of cells from different body sites can be helpful to select an optimal site for keratinocyte isolation before considering graft protocols for girls.

Epidermal healing in burns: autologous keratinocyte transplantation as a standard procedure: update and perspective

In the contexte of skin graft, cell suspensions transplanted directly to the wound is an attractive process, removing the need for attachment to a membrane before transfer and avoiding one potential source of inefficiency. Choosing an optimal donor site containing cells with high proliferative capacity is essential for graft success in burns.

Inhibition of keratinocyte differentiation by the synergistic effect of IL-17A, IL-22, IL-1α, TNFα and oncostatin M

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This study highlights the precise role of cytokines in the skin inflammatory response (psoriasis). IL-22 and OSM more specifically drive epidermal hyperplasia and differentiation loss while IL-1α, IL-17A and TNFα were more involved in the activation of innate immunity.
HNF1β prostate cancer

Expression patterns of candidate susceptibility genes HNF1β and CtBP2 in prostate cancer: Association with tumor progression

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Genome-wide association studies have identified variants at multiple loci associated with prostate cancer (PCa) risk. Some of these loci include candidate susceptibility genes, such as MSMB, HNF1β, and C-terminal-binding protein (CtBP2). Except for MSMB, the clinicopathological significance of these genes has not been investigated. We therefore aimed to analyze their expression in PCa tissues, in relation with tumor progression and aggressiveness.

Contribution of IL22 to experimental skin inflammation

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Focused on in vitro human models, we present the mechanisms of action of IL22 as well as its involvement in structure, metabolism, differentiation, chemotaxis, antibacterial activity, innate immunity, and tissue remodeling of epidermis.

Involvement of IL-1 and Oncostatin M in acanthosis associated with hypertensive leg ulcer

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Hypertensive leg ulcer (HLU) is an inflammatory disease characterized by intense pain, alteration of vascularization, and skin necrosis. The optimal treatment relies on surgical removal of necrotic tissues covered by a split-skin graft.

Foreskin-isolated keratinocytes provide successful extemporaneous autologous paediatric skin grafts

We report a successful method for grafting paediatric males presenting large severe burns through direct spreading of autologous foreskin keratinocytes. This alternative method is easy to implement, improves the quality of skin and minimizes associated donor site morbidity. in vitro studies have highlighted the potential of foreskin tissue for graft applications and could help in tissue selection with the prospect of grafting burns for girls.

Keratinocytes under fire of proinflammatory cytokines: Bonafide innate immune cells involved in the physiopathology of chronic atopic dermatitis and psoriasis

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Specific cytokine environment deregulation plays a central role on skin morphology and innate immunity, moving towards specific pathologies and opening the way to new therapeutic strategies.

Cystinosin is a melanosomal protein that regulates melanin synthesis

In vitro studies showed that cystinosin was located at melanosomes. CTNS silencing led to a 75% reduction of melanin synthesis that was caused by a degradation of tyrosinase by lysosomal proteases. Our results objectify the pigmentation defect in patients with cystinosis. We also identify the role of CTNS in melanogenesis and add a new gene to the list of the genes involved in the control of skin and hair pigmentation.
cytokine

Atopic dermatitis – initiation and chemokines activation step

The skin lesions facilitate the passage of an antigen that the subject has previously been sensitized to.
atopic dermatitis

Atopic dermatitis – immune response and skin lesions

TH2/TH22 immune response and other agents of atopic dermatitis After polarization, the Th2 and Th22 TL migrate to the lesion area, where they release type Th2 (IL-4, IL-5, IL-13, IL-31, TNF-α) and Th22 (IL-22) cytokines respectively. These Th2/Th22 cytokines have different functions in the immunity response.

The gap junction protein Cx43 is involved in the bone-targeted metastatic behaviour of human prostate cancer cells

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We investigated the effect of the increased Cx43 expression, by retroviral infection, on the metastatic behaviour of two well-characterized cell lines (PC-3 and LNCaP) representing different stages of PCa progression. It appeared that Cx43 differently behaved in those cell lines and induced different phenotypes.