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Immune Cells
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Human Mast Cell model
Primary cells
(human)
- Granulocytes (PMNs)
- Neutrophils
- Eosinophils
- Basophils
- T lymphocytes
- B lymphocytes
- Monocytes
- Macrophages
- Dendritic cells
- Mast cells
Cell lines
(human)
- U937
- THP1
- HL-60
- Jurkat
Ex vivo models
(human)
- skin explants
- hair explants
- Co-culture of human hair follicles with autologous primary immune cells (CD8+ T-cells, CD4+ T-cells, gamma delta T-cells, etc.)
In vivo models
- humanized-mouse models
Assays
- PMN, Macrophage, Dendritic Cell activation
- Phagocytosis
- PMN oxidative burst
- M1 or M2 polarization and switch
- Chemotaxis
- T cell differentiation
- Treg phenotyping
- Proliferation
- Cytokine and chemokine production and release
- Expression of cytokine and chemokine receptors
- Immune cell phenotype
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Regulation of skin and hair follicle physiology
- Number of immune cells in specific skin or hair follicle compartments