A novel clinical method to measure skin staining reveals activation of skin damage pathways by cigarette smoke
Cosmetics, Cosmetics, CosmétiqueAnti-pollution effects of two antioxidants and a chelator-Ex vivo electron spin resonance and in vivo cigarette smoke model assessments in human skin
CosmeticsType-1 cytokines regulate MMP-9 production and E-cadherin disruption to promote melanocyte loss in vitiligo
Pigmentation, PigmentationLoss of melanocytes is the pathological hallmark of vitiligo.
Profiling TLR agonist activity in keratinocytes and link to bacteria-induced innate immunity activation
Cosmetics, Microbiome cutané, Microbiome cutanéIn this study, our goal was first to functionally profile and characterize the response of normal human keratinocytes (NHEK) to microbial patterns (TLR agonists) and IL-1α in parallel. To do so, the effects of multiple TLR agonists and IL-1α were evaluated on the gene expression profile, on signal transduction (NF-ƘB translocation) and also on IL-8 and IL-6 release. Secondly, we also tried to link these responses to those of pathogens of interest in skin microbiota (namely, C. acnes, S. aureus and P. aeruginosa), by evaluating the effect on IL-8 release by NHEK infected with living bacteria from these 3 strains...
Comment réaliser des tests in vitro ? Amélioration de la qualité et de l’innocuité des cosmétiques
CosmeticsBehavior of the two circulating androgens testosterone and DHEA and their main intracellular metabolites in an androgen-sensitive sebocyte cell line.
Acne, Sebaceous gland regulationAndrogens are known to strongly regulate the sebaceous gland. Relationships with testosterone have been reported and little is known about the role of DHEA. We showed that the most potent intracellular androgen metabolite, dihydrotestosterone (DHT), induced AR translocation, specific transcripts such as ras-related dexamethasone induced 1 (RASD1) and lipogenesis/lipid storage. We used these parameters to assess the behaviour and activity of the two main circulating androgens TESTO and DHEA, in comparison with DHT, in this model.
Behavior of the two circulating androgens testosterone and DHEA and their main intracellular metabolites in an androgen-sensitive sebocyte cell line.
CosmeticsAndrogens are known to strongly regulate the sebaceous gland. Relationships with testosterone have been reported and little is known about the role of DHEA. We showed that the most potent intracellular androgen metabolite, dihydrotestosterone (DHT), induced AR translocation, specific transcripts such as ras-related dexamethasone induced 1 (RASD1) and lipogenesis/lipid storage. We used these parameters to assess the behaviour and activity of the two main circulating androgens TESTO and DHEA, in comparison with DHT, in this model.
Innate immunity activation of sebocyte cells by living bacteria. Evidence of a potential immunosuppressive effect of DHT.
Acne, Acné, Microbiome cutané, Microbiome cutané, Microbiome cutané, Sebaceous gland regulationAcne is a skin pathology targeting the pilosebaceous unit…sebum hypersecretion and bacterial infection. We studied the effects of living bacteria on the induction of an innate immune response in a sebocyte cell line.
Pharmacological effects of anti-androgens and other compounds in an androgen-sensitive sebocyte cell line.
Acné, Acne, Sebaceous gland regulationWe analysed the activity of reference anti-androgens (Finasteride, Dutasteride, Cyproterone acetate), at the level of their potential targets (5-alpha-reductase, AR translocation, induced transcripts) and in a functional lipid accumulation assay. We also evaluated this androgen-induced lipid accumulation assay as a potential pharmacological tool to detect potential inhibitors from both androgenic and non-androgenic origins.
Androgens activate lipogenesis through an AKT-independent mTOR pathway stimulation and a limitation of autophagy in an androgen-sensitive sebocyte cell line.
Acné, Acne, Peau grasse, hyperséborrhée et séborégulation, Sebaceous gland regulationAndrogens are key regulators of sebaceous function... A link between mTOR and androgen signaling has previously been reported...as well as a link between these processes and lipid synthesis...Here we aimed at confirming that DHT-induced lipid synthesis/accumulation is at least in part dependent on mTOR activation in the SEBO662AR cell line and that the autophagic process is consequently modified by the androgenic treatment.
Pollution and epigenetics: Histone H3 modifications in keratinocytes after exposure to urban dust and Benzo(a)pyrene
Cosmetics, Cosmetics, Cosmétique, Skin protection and immune defense systemIn this work, human epidermal keratinocytes were used to study the effects of urban dust and benzo(a)pyrene on the epigenetic pattern of the histone H3 protein. The assay was then used to screen a series of plant extracts in order to find cosmetic actives to protect the skin against air pollution.
Anti-inflammatory activity of CKC-containing cationic emulsion eye drop vehicles
Cosmetics, Inflammation, PharmacologyPreservative-free cationic emulsion-based artificial tears (ATs) or drug vehicles are innovative eye drop formulations with tear film stabilization and drug delivery properties, and valuable in vivo anti-inflammatory and wound healing properties.
New Biological Activities of Lythrum salicaria L.
Barrière cutanée et hydratation, Cosmetics, Cosmétique, Skin barrier and hydrationThe perennial and widespread herb Lythrum salicaria L., also called purple loosestrife, is a plant that is traditionally used in European medicine.
Effects of different kinds of pollutants on the lipidic metabolism of human sebocytes and protective effects of a new macroalgae culture extract
Cosmetics, Peau grasse, hyperséborrhée et séborégulation, Protection et défenses cutanées, Sebaceous gland regulation, Skin protection and immune defense systemSebocytes lipid production are stimulated by pollutants. Ame acts by protecting epidermal cells, human sebocytes and skin from urban dust.
A Compensating Skin Care Complex Containing Pro-xylane in Menopausal Women: Results from a Multicentre, Evaluator-blinded, Randomized Study
Cosmetics, Cosmetics, CosmétiqueIn addition to the natural process of skin aging, the skin of women is significantly impacted during and after menopause.
New biological effects of Aphloïol
Barrière cutanée et hydratation, Cosmetics, Cosmétique, Skin barrier and hydrationIn the present study, we identified new properties of Aphloïol (syn. mangiferin) in improving skin barrier function, especially by promoting keratinocyte differentiation, synthesis of transmembrane glycoprotein and lipid neosynthesis.
in vitro modeling of skin photoaging: development of evaluation tools for cosmetics
CosmeticsDevelopment of in vitro models skin for better understand the modifications during photo-aging induced by repetitive UV exposure.
in vitro modeling of skin photoaging: development of evaluation tools for cosmetics
Cosmetics, Skin ageing, Vieillissement cutanéDevelopment of in vitro models skin for better understand the modifications during photo-aging induced by repetitive UV exposure.