Hair follicle, gene expression (cytokine mix)
FOLL-0017- Context
- Descriptions
- Requirement
- Study and reporting
- See also
Certain types of biological malfunctions may have an effect over the growth cycle of the hair follicle and will lead to premature hair loss (alopecia). They may be hormone-related (androgynous alopecia) or caused by an autoimmune disorder (alopecia areata). Alopecia areata is characterized by the initiation of an autoimmune reaction against the intrafollicular part of the hair follicle during the anagen phase of the growth cycle. This reaction is linked to the infiltration of T lymphocytes and the release of primarily Th2/Th22 cytokines, as in atopic dermatitis. This inflammatory reaction induces gene expression modulation and causes reduction of hair growth and then a premature entry into the telogen phase, thus preventing regrowth once the follicle has been shed. This in vitro assay consists in evaluating, via RT-qPCR and in micro dissected human hair follicles, the effect of compounds on a panel of genes modulated by alopecic inflammation (inflammation, extracellular matrix, proteases).
| Biological Model | Hair follicle (Dissect) |
|---|---|
| Species | Human |
| EndPoint | mQPA-HAIR-DA-8 |
| Method | qPCRarray (standard or customized) |
| Stimulation Factor | IL-4 (10 ng/ml) + IL-13 (10 ng/ml) + TNF- alpha (10 ng/ml) + IL-22 (10 ng/ml) |
| Compound Reference | Jak inhibitor 1 |
| Physiopathology | Skin and cutaneous appendices > Skin inflammation and disorders > Atopic dermatitis |
Feasibility, study proposal:
Our sales team is here to help you find solutions:
Price: our pricing system consists of a fixed price per study + pricing per unit (price per replicate or experimental condition)
Start of assays and turnaround time: the start of an assay is subject to the receipt of your agreement and of the compounds to be tested. The sending date of the study report is communicated by email when the study starts. Although of an average of 5 to 10 weeks, our turnaround time may be subject to some fluctuation (due to availability of biological samples, to workload, etc.).
Required quantity of compounds: the required quantity is generally of a few mg or ml but may vary according to the form and solubility of compounds and to the concentrations to be tested. Please contact us for an estimation.
Customized protocols: for this assay, as for all of our assays, we can develop a protocol adapted to your needs. Should you have any question, please contact us.
Our sales team is here to help you find solutions:
- Technical feasibility study of your project
- Development of an assay selection strategy
- Definition of an experimental scheme
- Financial assessment and contractual agreements (costs, turnaround time, non-disclosure agreements, etc.)
Price: our pricing system consists of a fixed price per study + pricing per unit (price per replicate or experimental condition)
Start of assays and turnaround time: the start of an assay is subject to the receipt of your agreement and of the compounds to be tested. The sending date of the study report is communicated by email when the study starts. Although of an average of 5 to 10 weeks, our turnaround time may be subject to some fluctuation (due to availability of biological samples, to workload, etc.).
Required quantity of compounds: the required quantity is generally of a few mg or ml but may vary according to the form and solubility of compounds and to the concentrations to be tested. Please contact us for an estimation.
Customized protocols: for this assay, as for all of our assays, we can develop a protocol adapted to your needs. Should you have any question, please contact us.
Study management: each project requires advice and expertise so each study is managed and monitored by a dedicated study director who will be attentive to all your needs and questions.
Validation: the assays listed in the catalogue have undergone a preliminary validation and are subject to Standard Operating Procedures (SOPs) and to validation criteria. However, the robustness of each assay may be different according to the models and to the analytical methods. Please do not hesitate to contact us for more information.
Report and experimental data: our services include the writing of a study report in English or in French. This scientific report is provided in a PDF or Microsoft® Office format.
Laboratory / Location: this assay (as all of our assays) will be carried out in our Gençay laboratory in France.
Validation: the assays listed in the catalogue have undergone a preliminary validation and are subject to Standard Operating Procedures (SOPs) and to validation criteria. However, the robustness of each assay may be different according to the models and to the analytical methods. Please do not hesitate to contact us for more information.
Report and experimental data: our services include the writing of a study report in English or in French. This scientific report is provided in a PDF or Microsoft® Office format.
Laboratory / Location: this assay (as all of our assays) will be carried out in our Gençay laboratory in France.
