NHEK, mitochondrial membrane potential (JC-1 Dye labeling) (protection against urban dust)
NHEK-0264- Context
- Descriptions
- Requirement
- Study and reporting
- See also
The purpose of this in vitro assay is to evaluate, via imagery, the protective effects of compounds (molecules, active ingredients, extracts) on the mitochondrial depolarization induced by a pollutant (SMR1649b urban dust)
There is evidence today that mitochondria are one of the main targets of environmental toxic substances (pollution) which can damage mitochondrial morphology and function, thus resulting in the acceleration of cell ageing and even in the development of certain pathological disorders ( e.g. metabolic syndrome).
Mitochondria play a central role in the production of ATP and metabolic cofactors (NADH, FADH2) involved in the respiratory chain. They are also involved in key processes linked to cellular differentiation and to the regulation of the cellular cycle.
In order to assess cell health, the study of membrane potential can be a discriminating indicator of the functional state of cells.
Here, the cyanine dye JC-1 (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide) facilitates the discrimination of “energized” and “deenergized” (depolarized) mitochondria as the normally green fluorescent dye forms red fluorescent aggregates when it is concentrated in “energized” mitochondria, as a response to their higher membrane potential.
| Biological Model | NHEK (Normal Human Epidermal Keratinocytes) |
|---|---|
| Species | Human |
| EndPoint | Mitochondrial membrane potential (JC-1 Dye labeling) |
| Method | Fluorescence / imaging |
| Stimulation Factor | Urban dust (SRM, 1649b) |
| Physiopathology | Skin and cutaneous appendices > Epidermal protection against stress conditions and defense reinforcement > Pollution |
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Start of assays and turnaround time: the start of an assay is subject to the receipt of your agreement and of the compounds to be tested. The sending date of the study report is communicated by email when the study starts. Although of an average of 5 to 10 weeks, our turnaround time may be subject to some fluctuation (due to availability of biological samples, to workload, etc.).
Required quantity of compounds: the required quantity is generally of a few mg or ml but may vary according to the form and solubility of compounds and to the concentrations to be tested. Please contact us for an estimation.
Customized protocols: for this assay, as for all of our assays, we can develop a protocol adapted to your needs. Should you have any question, please contact us.
Validation: the assays listed in the catalogue have undergone a preliminary validation and are subject to Standard Operating Procedures (SOPs) and to validation criteria. However, the robustness of each assay may be different according to the models and to the analytical methods. Please do not hesitate to contact us for more information.
Report and experimental data: our services include the writing of a study report in English or in French. This scientific report is provided in a PDF or Microsoft® Office format.
Laboratory / Location: this assay (as all of our assays) will be carried out in our Gençay laboratory in France.
