1 – IL-1fm gene expression in keratinocytes
2 – IL-1fm protein expression in keratinocytes
CONCLUSIONS
Through the modeling of the epidermal lesion with a standardized keratinocyte extract, we have shown that fibroblasts are the most sensitive cell type among all other tested skin resident cells.
Stimulation of fibroblasts with keratinocyte extract induced a strong inflammatory response which we have demonstrated to be mediated by IL-1α.
Whereas fibroblasts are extremely sensitive to IL-1, they are overall insensitive to IL-1Ra, which is yet present in massive amounts in the keratinocyte extract.
On the contrary, IL-1Ra highly protects keratinocytes from IL-α stimulation.
Altogether these data indicate that fibroblasts may act as local sensors of primary epidermis lesion through keratinocyte IL-1α release.
In this context, IL-1Ra released together with IL-1α, should protect other skin cells from activation and limit the inflammatory response.
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