Immuno-Inflammation - QIMA Life Sciences

IMMUNE-INFLAMMATION

Research areas

Cell and tissue damage

Acute inflammation or stress: innate immunity & epithelial barrier response against damage
Defense mechanisms
Chronic inflammation, stress or disease: tissue response, damage, ageing, etc.

Regulation of immune and inflammatory responses

Recruitment, proliferation, differentiation, polarization and function of immune cells
Cytokine, chemokine and growth factor release

Tissue Regeneration

Tissue repair, wound healing
Regeneration, proliferation and migration
Differentiation process
Function and recovery

Immune blood cells

Primary cells (human)

Whole blood
PBMC
PMN
CD4+ T lymphocytes
CD19+ B lymphocytes B
CD14+ monocytes , M1 and M2 macrophages
Dendritic and langerhans cells
Granulocytes
Basophils

Primary cells (murin)

Splenocytes
Thymocytes
Mast cells

Cell lines

U937
THP1
HL-60
Jurkat

in vitro pharmacology assays using blood/immune cells

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IMMUNE-INFLAMMATION

Research areas

Immune blood cells

in vitro pharmacology assays using blood/immune cells

Activated M2 macrophages, Switch towards M1 macrophages (basal)

Blood, CD11b/CD62L expression (TNF- alpha stimulation)

Blood, H2O2 content (PMA stimulation)

Blood, human basophil activation assay (BAT, basal)

Blood, human basophil activation assay (BAT, fMLP stimulation)

Blood, particle phagocytosis

CD14+ monocytes, Cytokine release (basal)

CD14+ monocytes, cytokine release (LPS stimulation)

CD14+ monocytes, cytokine release (LPS stimulation)

CD14+ monocytes, M1-type polarized macrophages, Phenotyping and cell viability (basal)

CD14+ monocytes, M2-type polarized macrophages, Phenotyping and cell viability (basal)

CD19+ B cells, Ig production (basal)

CD19+ B cells, Ig production (IL-21 stimulation)

CD19+ B cells, IL-6 release (anti-CD40+IL-4 stimulation)

CD19+ B cells, phenotyping & proliferation (basal)

CD4+ T cells, cytokine release (anti-CD3/anti-CD28 stimulation)

CD4+ T cells, cytokine release (anti-CD3/anti-CD28 stimulation)

CD4+ T cells, Th1 cytokine release (anti-CD3/anti-CD28 stimulation)

CD4+ T cells, Th17 cytokine release (anti-CD3/anti-CD28 stimulation)

CD4+ T cells, Th2 cytokine release (anti-CD3/anti-CD28 stimulation)

HL-60, arachidonic acid metabolite release (A23187 stimulation)

HL-60, CAMP (LL-37 precursor) mRNA expression (basal)

HL-60, IL-8 release (PMA stimulation)

M1 macrophages, Phenotyping and/or Cytokine release (IFN- gamma + LPS stimulation)

Mast cells degranulation, histamine release (compound 48/80 stimulation)

Mo-DC, cytokine release (basal)

Mo-DC, cytokine release (LPS stimulation)

Mo-LC, cytokine release (PGN stimulation)

Monocytes, opioid receptor labeling (Basal)

PBMC, anti-CD3/anti-CD28-stimulated T cells, cytokine release (anti-CD3/anti-CD28 stimulation)

PBMC, anti-CD3/anti-CD28-stimulated T cells, cytokine release (anti-CD3/anti-CD28 stimulation)

PBMC, anti-CD3/anti-CD28-stimulated T cells, Th1 cytokine release (anti-CD3/anti-CD28 stimulation)

PBMC, anti-CD3/anti-CD28-stimulated T cells, Th17 cytokine release (anti-CD3/anti-CD28 stimulation)

PBMC, anti-CD3/anti-CD28-stimulated T cells, Th2 cytokine release (anti-CD3/anti-CD28 stimulation)

PBMC, anti-CD3/anti-CD28-stimulated T cells, Th22 cytokine release (anti-CD3/anti-CD28 stimulation)

PBMC, IFN- gamma release (anti-CD28 stimulation)

PBMC, inflammation gene expression (LPS stimulation)

PBMC, LPS-stimulated monocytes, cytokine release (LPS stimulation)

PBMC, LPS-stimulated monocytes, cytokine release (LPS stimulation)

PBMC, O2- content (PMA stimulation)

PBMC, PI3K/AKT/mTOR or MAPkinases activation (Basal)

PBMC, T cell proliferation (Two-Way Mixed Lymphocyte Reaction)

PMN, CD11b/CD62L expression (TNF- alpha stimulation)

PMN, cytokine release (LPS stimulation)

PMN, H2O2 content (PMA stimulation)

PMN, IL-8 release (TNF- alpha stimulation)

PMN, migration (basal)

PMN, O2- content (PMA stimulation)

PMN, particle phagocytosis (basal)

Skin maintained ex vivo, DC/LC immunolabeling (basal)

Splenocyte proliferation (basal)

T cell proliferation (PHA stimulation)

T cells, CD25 expression (PHA stimulation)

T cells, IFN- gamma release (anti-CD3 stimulation)

T cells, IFN- gamma release (basal)

THP-1 (differentiated), cytokine release (LPS stimulation)

THP-1, caspase 1, enzymatic activity (basal)

Thymocytes, caspase 3 (basal)

Thymocytes, caspase 3 (glucocorticoid stimulation)

U-937 apoptosis (basal)

U-937, arachidonic acid metabolite release (LPS stimulation)

U-937, cytokine/chemokine release (LPS stimulation)

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