Acne is a chronic inflammatory pathology of the pilosebaceous follicle under hormonal dependence that includes:
- abnormal increase of sebaceous lipid production (sebum)
- formation of microcomedones due to an abnormal epidermal differentiation, a likely consequence of abnormal androgen (testosterone) metabolism
- accumulation of peroxidized lipids on the surface (blackheads) or inside the comedo (whitehead)
- formation of infectious and non-infectious inflammatory lesions and a skin wound healing process
Acne: in vitro models and assays
QIMA Life Sciences has many in vitro or ex vivo models related to acne at your disposal:
- Human sebocytes: SEBO662 and SEBO662AR cell lines
- Normal human epidermal keratinocytes (NHEK)
- Normal human dermal fibroblasts (NHDF)
- Human follicle dermal papilla cells (HFDPC)
- Reconstructed human epidermis (RHE)
QIMA Life Sciences provides you with a range of innovative in vitro pharmacological models and assays, more particularly dedicated to the evaluation of the pharmacological efficacy (screening, profiling, proof-of-concept) of your products (APIs, biosimilars, fomulations, medical devices):
- Sebaceous gland regulation and hyperseborrhea
- Androgen metabolism
- Abnormal epidermal differentiation
- Lipid peroxidation
- Inflammatory, lesional or microbial response
- Infection, immune system and skin defenses
Here are a few examples among all assays proposed by QIMA Life Sciences in the field of acne:
Innate immunity activation of sebocyte cells by living bacteria. Evidence of a potential immunosuppressive effect of DHT.Acne, Microbiome cutané, Sebaceous gland regulation, Skin microbiome, Skin microbiome
Acne is a skin pathology targeting the pilosebaceous unit…sebum hypersecretion and bacterial infection. We studied the effects of living bacteria on the induction of an innate immune response in a sebocyte cell line.
Pharmacological effects of anti-androgens and other compounds in an androgen-sensitive sebocyte cell line.Acne, Sebaceous gland regulation
We analysed the activity of reference anti-androgens (Finasteride, Dutasteride, Cyproterone acetate), at the level of their potential targets (5-alpha-reductase, AR translocation, induced transcripts) and in a functional lipid accumulation assay. We also evaluated this androgen-induced lipid accumulation assay as a potential pharmacological tool to detect potential inhibitors from both androgenic and non-androgenic origins.
Androgens activate lipogenesis through an AKT-independent mTOR pathway stimulation and a limitation of autophagy in an androgen-sensitive sebocyte cell line.Acne, Peau grasse, hyperséborrhée et séborégulation, Sebaceous gland regulation
Androgens are key regulators of sebaceous function… A link between mTOR and androgen signaling has previously been reported…as well as a link between these processes and lipid synthesis…Here we aimed at confirming that DHT-induced lipid synthesis/accumulation is at least in part dependent on mTOR activation in the SEBO662AR cell line and that the autophagic process is consequently modified by the androgenic treatment.
Acne vulgaris: Origins, symptoms and current treatmentsAcne
Acne vulgaris is a chronic inflammatory disease of the skin which affects most adolescents and is the cause of over half of all dermatology consultations. This pathology, which is characterized by a hypersecretion of sebum, can be the cause of physical complications and may also lead to psychological distress.
Acne vulgaris: physiopathology and cell mechanismsAcne, Sebaceous gland regulation
Acne Vulgaris is a chronic inflammatory pathology located in the region of the pilosebaceous follicle. It occurs following a high production of sebum linked to hormonal (androgynous) or environmental (pollution) malfunctioning and to the colonization of the pilosebaceous follicle by certain bacteria (P. acnes).
QIMA Life Sciences has developed a panel of innovative assays to discover and evaluate compounds for the treatment of acne.
Physiology and functions of the sebaceous glandAcne, Sebaceous gland regulation, Skin barrier and hydration
The sebaceous gland is an organ located in the dermis. Its role is to synthesise and secrete sebum which is a component of the hydrolipidic film. The purpose of sebum is to protect the skin from external aggression and dehydration. Sebum also maintains the suppleness of the skin and hair.
A skincare combined with combination of adapalene and benzoyl peroxide provides a significant adjunctive efficacy and local tolerance benefit in adult women with mild acneAcne, Acné
Acne in adult women is an increasing reason for dermatological consultations.
Androgens induce sebaceous differentiation in sebocyte cells expressing a stable functional androgen receptorAcne, Cell and tissue engineering, Sebaceous gland regulation
In order to study the impact of active androgens in sebocytes, we constructed a stable human sebocyte cell line derived from SEBO662  constitutively expressing a fully functional AR. In these SEBO662 AR+ cells, dihydrotestosterone (DHT) induced AR nuclear translocation and the strong modulation of a set of transcripts (RASD1, GREB1…) known to be androgen-sensitive in other androgenic cells and tissues.
Immortalized sebocytes SEBO662 can spontaneously differentiate into a sebaceous-like phenotype when cultured as a 3D epitheliumAcne, Cell and tissue engineering, Sebaceous gland regulation
SEBO662 multilayers spontaneously differentiate into a sebaceous-like structure as shown by the strong polarized expression of the late sebaceous marker EMA, the overexpression of some lipogenic markers and lipid production on the upper side of the epithelium.
Keratinocytes as targets for cytokines in skin inflammationAcne, Atopic dermatitis, Dermatology, Psoriasis
Current knowledge about the effects of different cytokine families on keratinocytes, and more particularly theirinvolvement in skin inflammation and in the development of inflammatory skin diseases such as psoriasis
Expression of type 1 5-alpha-reductase and metabolism of testosterone in reconstructed human epidermisAcne, Dermatology
We analysed the potential of a human epidermal tissue reconstituted by cell culture (RHE, provided by SkinEthic Laboratories, Nice, France) as a model for assessing 5-alphaR activity.