QIMA Life Sciences 提供许多可任由您自由支配的体外模型:
- 内在老化模型:
- “老年”人真皮成纤维细胞(Hayflick模型)
- “老年”人皮肤等效物(Hayflick模型)
- 加速老化模型:
- 氧化应激老化的人类真皮成纤维细胞(H2O2)
- 缺乏培养基中的人体重建皮肤
- 光老化模型:
- 人皮肤成纤维细胞经受UVA,红外或UV辐射
- 光老化人全厚度重建皮肤
在这些模型上,我们可通过衡量以下项来评估活性化妆品化合物的抗衰老效果:
- 细胞更新(细胞增殖,迁移和分化)
- 细胞外基质的合成和降解(胶原蛋白,弹性蛋白,透明质酸,MMPs等)
- 衰老标记表达
- 自由基产生
以下是 QIMA Life Sciences 在皮肤老化领域提出的所有标准测定中的几个例子:
in vitro modeling of skin photoaging: development of evaluation tools for cosmetics
Cosmetics_cat_publi, Skin ageingDevelopment of in vitro models skin for better understand the modifications during photo-aging induced by repetitive UV exposure.
Development of a new model of reconstructed aged skin useful to study antiageing effects of cosmetic compounds
Cell and tissue engineering, Skin ageingThe development of new anti-ageing products needs performant in vitro models mimicking morphological changes and physiological modifications appearing during skin ageing. In order to have access to a simple model mimicking the epidermis ageing but in relation with a normal dermis, we have developed a new in vitro model of reconstructed skin comprising an aged epidermis covering a reconstructed dermis built with collagen and normal (young) fibroblasts.
Effect of C-xyloside on morphogenesis of the dermal epidermal junction in aged female skin. An ultrastuctural pilot study
Cosmetics_cat_publi, Cosmétique, Skin ageingThese data suggest that topical C-xyloside application in vivo may be efficient in inducing a better dermal-epidermal cohesion when such a junction is deficient, as is the case in photo-aged or chronologically aged skin. Moreover, a statistically significant increase in CD44 expression was noted in the epidermis of C-xyloside-treated compared to the placebo treated skin areas.
Reduced expression of the adhesion protein tensin1 in cultured human dermal fibroblasts affects collagen gel contraction
Cosmetics_cat_publi, Cosmétique, Skin ageingtensin1 plays an important role in cell-matrix interaction and that a reduced expression might contribute to the dermal alterations observed during skin ageing