QIMA Life Sciences 有许多体外或离体模型可供您使用:
- 正常人表皮角质形成细胞(NHEK)
- 正常人皮肤成纤维细胞(NHDF)
- 重建人表皮(RHE)
- 正常,光老化和缺乏的人体全层再造皮肤(FTSK)
- 皮肤外植体(离体)
在这些模型上,我们可通过衡量以下项来评估活性成分或制剂的保护效果:
- 红外线,UVB,UVB +(A)辐射
- 自由基
- 污染(城市灰尘,室内灰尘,香烟烟雾等)
- 化学应激(PMA,SDS等)
- 微生物(细菌,酵母,病毒等)或与病原体相关的分子模式(TLR 激动剂等)
- 渗透压,热应力等
- 营养缺乏
通过测量(例如):
- 细胞活力或代谢
- 炎症介质的释放(IL-8,PGE2,IL-6,TNF-α 等)
- 自由基产生
- DNA损伤(CPD,晒伤细胞,彗星试验等)
- 金属蛋白酶的表达或产生
- 抗菌肽产生(β-防御素,S100A7 等)
这里是 QIMA Life Sciences 在皮肤保护和防御领域提出的所有测定方法中的几个例子:
Pollution and epigenetics: Histone H3 modifications in keratinocytes after exposure to urban dust and Benzo(a)pyrene
Cosmetics, Cosmetics, Cosmétique, Skin protection and immune defense systemIn this work, human epidermal keratinocytes were used to study the effects of urban dust and benzo(a)pyrene on the epigenetic pattern of the histone H3 protein. The assay was then used to screen a series of plant extracts in order to find cosmetic actives to protect the skin against air pollution.
Effects of different kinds of pollutants on the lipidic metabolism of human sebocytes and protective effects of a new macroalgae culture extract
Cosmetics, Peau grasse, hyperséborrhée et séborégulation, Sebaceous gland regulation, Skin protection and immune defense systemSebocytes lipid production are stimulated by pollutants. Ame acts by protecting epidermal cells, human sebocytes and skin from urban dust.