皮肤微生物群：体外模型和检测
QIMA Life Sciences 拥有细胞和组织工程方面的专业知识，已经开发出多种体外或离体研究模型，适用于分析微生物群与皮肤之间的相互作用：
- 隔离式细菌培养（金黄色酿脓葡萄球菌、表皮葡萄球菌、疮疱丙酸杆菌）
- 利用 HSV-1 病毒进行感染实验（单纯疱疹病毒 I 型）
- 共培养：
- 2D 皮肤模型上的细菌（角质细胞、成纤维细胞、痤疮丙酸杆菌）
- 3D 皮肤模型上的细菌（外植体、重建表皮等）
化合物或配方对细菌发育的影响以及细菌对皮肤反应的影响都可以通过这些模型进行评估。
QIMA Life Sciences 可提供一组解决方案，用于评估：
- 细菌活力
- 菌落量化 (CFU)
- 细菌负荷测定 (qPCR (Taqman))
- 3D 模型上的细菌黏附（外植体、重建表皮）
- 2D 或 3D 模型的生物反应（分化、发炎和免疫反应）
以下是 QIMA Life Sciences 在皮肤微生物群领域提出的所有标准化验的几个例子：
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The Relationship Between Microbiology Testing and the Skin Microbiota
Cosmetics, Skin microbiomeMicrobiology testing is essential for substantiating microbiome-friendly and anti-microbial claims. Read on to find out how cosmetic microbiology testing is connected to the skin microbiome.
Microbiota vs. Microbiome: What’s the Difference?
Skin microbiomeWhat is the difference between microbiota vs. microbiome? Why are they important to human skin? What is the Skin Microbiota test? Learn more here.
Using Reconstructed Human Epidermis model in Skin Microbiome Research
Skin microbiome, Skin microbiomeSkin microbiome research is developing rapidly. Are you up to date? Find out why 3D skin models are an important development in skin microbiome research.
Profiling TLR agonist activity in keratinocytes and link to bacteria-induced innate immunity activation
Cosmetics, Microbiome cutané, Skin microbiomeIn this study, our goal was first to functionally profile and characterize the response of normal human keratinocytes (NHEK) to microbial patterns (TLR agonists) and IL-1α in parallel. To do so, the effects of multiple TLR agonists and IL-1α were evaluated on the gene expression profile, on signal transduction (NF-ƘB translocation) and also on IL-8 and IL-6 release. Secondly, we also tried to link these responses to those of pathogens of interest in skin microbiota (namely, C. acnes, S. aureus and P. aeruginosa), by evaluating the effect on IL-8 release by NHEK infected with living bacteria from these 3 strains…