Lipolytic

Lipolytic effect of a polyphenolic citrus dry extract of red orange, grapefruit, orange (Sinetrol) in human body fat adipocytes. Mechanism of action by inhibition of cAMP-Phosphodiesterase (PDE)

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The present study investigated the lipolytic (break of fat stored) effect of a citrus-based polyphenolic dietary supplement (SINETROL) at human adipocytes (ex vivo), body fat (clinical) and biochemical levels (inhibition of phosphodiesterase).
tensin1

Reduced expression of the adhesion protein tensin1 in cultured human dermal fibroblasts affects collagen gel contraction

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tensin1 plays an important role in cell-matrix interaction and that a reduced expression might contribute to the dermal alterations observed during skin ageing
UV-induced

Alternative (non-animal) methods for cosmetic testing: current status and future prospects – UV-induced effects

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Cosmetic ingredients and mixtures of ingredients absorbing UV light (in particular UV filter chemicals used, for example, to ensure the light stability of cosmetics or used in sun protection products) should be tested for acute phototoxic and photogenotoxic potential.
acute toxicity

Alternative (non-animal) methods for cosmetic testing: current status and future prospects – Acute toxicity

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From the regulatory point of view, the main objective of acute toxicity testing is basically to classify chemicals according to their intrinsic toxicity
gene expression

Comparison of gene expression profiles in human keratinocyte mono-layer cultures, reconstituted epidermis and normal human skin; transcriptional effects of retinoid treatments in reconstituted human epidermis

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In order to validate a model for predictive screening of dermatological drugs, we used a customized cDNA macro-array system containing 475 skin-related genes to analyze the gene expression patterns in human keratinocytes from different origins.
phototoxicity

Development of a highly sensitive in vitro phototoxicity assay using the SkinEthic reconstructed human epidermis

The reconstituted human epidermis model SkinEthic was used to evaluate the phototoxicity of topically applied chemicals.