GPDB: Chemical Compound Structure Database

Ethnopharmacologic database -GPDB

,

in silico screening – SELNERGY

,
in vitro and ex vivo assays: skin ageing

How is In Vitro Testing Done? Improve Quality & Safety of Cosmetics

Photometry

5 Key Questions to Ask When Choosing a Preclinical Dermatology CRO

Photometry

In Vitro Testing in Cosmetics: Pros and Cons

Photometry

How are In Vitro Testing Methods Used for Cosmetics?

,
With the growing distaste and outright bans of animal testing of cosmetic products, more and more cosmetics companies are turning to in vitro testing. Let’s take a closer look at the nature of in vitro testing and the opportunities it offers for cosmetics brands and manufacturers.

Atopic dermatitis

AD is the most common dermatosis affecting children: 65% of the patients are less than a year old and 85% are below 5 years. The prevalence of this pathology is constantly on the rise and currently affects 10 to 25% of the population.

Sample and slide preparation

Human-skin

Histological staining

Behavior of the two circulating androgens testosterone and DHEA and their main intracellular metabolites in an androgen-sensitive sebocyte cell line.

Androgens are known to strongly regulate the sebaceous gland. Relationships with testosterone have been reported and little is known about the role of DHEA. We showed that the most potent intracellular androgen metabolite, dihydrotestosterone (DHT), induced AR translocation, specific transcripts such as ras-related dexamethasone induced 1 (RASD1) and lipogenesis/lipid storage. We used these parameters to assess the behaviour and activity of the two main circulating androgens TESTO and DHEA, in comparison with DHT, in this model.

Innate immunity activation of sebocyte cells by living bacteria. Evidence of a potential immunosuppressive effect of DHT.

, , , ,
Acne is a skin pathology targeting the pilosebaceous unit…sebum hypersecretion and bacterial infection. We studied the effects of living bacteria on the induction of an innate immune response in a sebocyte cell line.

Pharmacological effects of anti-androgens and other compounds in an androgen-sensitive sebocyte cell line.

,
We analysed the activity of reference anti-androgens (Finasteride, Dutasteride, Cyproterone acetate), at the level of their potential targets (5-alpha-reductase, AR translocation, induced transcripts) and in a functional lipid accumulation assay. We also evaluated this androgen-induced lipid accumulation assay as a potential pharmacological tool to detect potential inhibitors from both androgenic and non-androgenic origins.

Androgens activate lipogenesis through an AKT-independent mTOR pathway stimulation and a limitation of autophagy in an androgen-sensitive sebocyte cell line.

, ,
Androgens are key regulators of sebaceous function... A link between mTOR and androgen signaling has previously been reported...as well as a link between these processes and lipid synthesis...Here we aimed at confirming that DHT-induced lipid synthesis/accumulation is at least in part dependent on mTOR activation in the SEBO662AR cell line and that the autophagic process is consequently modified by the androgenic treatment.
in vitro and ex vivo assays: skin protection and immune defense system

Pollution and epigenetics: Histone H3 modifications in keratinocytes after exposure to urban dust and Benzo(a)pyrene

, , ,
In this work, human epidermal keratinocytes were used to study the effects of urban dust and benzo(a)pyrene on the epigenetic pattern of the histone H3 protein. The assay was then used to screen a series of plant extracts in order to find cosmetic actives to protect the skin against air pollution.
Dog Skin - Epidermal Cornification Proteins

Refined Immunochemical Characterization in Healthy Dog Skin of the Epidermal Cornification Proteins, Filaggrin, and Corneodesmosin

, , ,
Filaggrin (FLG) and corneodesmosin (CDSN) are two key proteins of the human epidermis.

Oncostatin M is overexpressed in skin squamous-cell carcinoma and promotes tumor progression

Cutaneous squamous cell carcinoma (cSCC) is the second most common keratinocyte malignancy and accounts for 20% of skin cancer deaths.
Pollution-Induced Skin Damage

Clinical Efficacy of a Novel Two-Part Skincare System on Pollution-Induced Skin Damage

Air pollution continues to be a global health concern and recent studies have shown that air pollutants can cause skin damage and skin aging through several pathways that induce oxidative stress, inflammation, apoptosis, and skin barrier dysfunction.