A novel clinical method to measure skin staining reveals activation of skin damage pathways by cigarette smoke
Skin Res Technol. 2021;1-9
DALRYMPLE A., MCEWAN M., BRANDT M., BIELFELDT S., BEAN EJ., MOGA A., COBURN S., HARDIE G. (2021)
British American Tobacco, R&D, Southampton, Hampshire, UK.
proDERM, Institut für Angewandte Dermatologische Forschung, Hamburg,Germany.
Synelvia, Labège, France.
Background: Long-term use of cigarettes can result in localised staining and aging of smokers’ skin. The use of tobacco heating products (THPs) and electronic cigarettes (ECs) has grown on a global scale; however, the long-term effect of these products’ aerosols on consumers’ skin is unknown. This pilot clinical study aimed to determine whether THP or EC aerosol exposure results in skin staining or activation of biomarkers associated with oxidative stress.
Materials and methods: Eight areas were identified on the backs of 10 subjects. Two areas were used for air control, and two areas exposed to 32-puffs of cigarette smoke (CS), THP or EC aerosols, which were delivered to the skin using a 3-cm diameter exposure chamber and smoke engine. Skin colour was measured using a Chromameter. Squalene (SQ), SQ monohydroperoxide (SQOOH) and malondialdehyde (MDA) levels were measured in sebum samples by mass spectrometry and catalase colorimetry.
Results: CS exposure significantly increased skin staining,SQOOHandMDAlevels and SQOOH/SQratio. THPand ECvalueswere significantly lower thanCS;ECvalues being comparable to air control. THP valueswere comparable to ECand air control at all endpoints, apart from skin staining. SQ and catalase levels did not change with exposure.
Conclusions: CS stained skin and activated pathways known to be associated with skin damage. THPs and ECs produced significantly lower values, suggesting they could offer hygiene and cosmetic benefits for consumers who switch exclusively from smoking cigarettes. Further studies are required to assess longer-term effects of ECs and THPs on skin function.
© 2021 John Wiley & Sons Ltd.
KEYWORDS: cigarette smoke, cosmetic, electronic cigarette/e-cigarette, hygiene, skin damage, skin staining, tobacco heating product